n gene meaning in Chinese
n基因
Examples
- However , the homologies of g and n gene between wild strains and attenuated strain ctn are much high
三株野毒与减毒株ctn之间的n和g基因同源性均高于与3ag 、 era株的同源性。 - 97 % identities in amino acids respectively . the e . coli strain dh5 transformed recombinant plasmid phn was induced with 0 . 6 mmol / m iptg for n gene expression . the expressed product was identified by sds - page and westem - blot test , a fusion protein about 47ku as we expected was found
将含有重组质粒phn的菌株dh5在37条件下培养，以浓度为0 . 6mmol / liptg诱导，重组质粒n基因phn融合蛋白获得了表达：经sds - page ， western - blot试验，确定其表达的融合蛋白产物大小为预期的47ku 。 - Ln our experiment , a lot of regulator sequences inc1uding the 35s promoter with double enhancer e1ements and the terminator nos , the fl sequence and kozak sequence for improving the genetic statement in the level of translation , were appended on the two s ides of the artifically pseudopleuronectes americanus antifreeze protei n gene by a series of process , and the products were linked into pbil21
本实验中人工合成了美洲拟鲽抗冻蛋白基因，并在美洲拟鲽抗冻蛋白基因两侧添加了许多调控序列，如具有加倍增强子的35s启动子和nos终止子，它们可以促进抗冻蛋白基因的转录，及可在翻译水平上提高基因表达的序列和kozak序列，从而构建了抗冻蛋白基因的高效表达盒。 - In this paper , a field strain of infectious bronchitis virus was isolated from proventriculus tissue , morphological observation by electron - microscope and the biological characterizations of the virus were studied , pairs of specific primers are designed and synthesized in correspondence with them , according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes , the cdna of si gene , s2 gene , m gene . n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported
在此基础上，根据国内外已发表的ibv基因序列，分别设计特异性引物，应用不同引物进行反转录合成cdna ，分片段对ibv的主要结构基因进行pcr扩增，并分别将各个目的片段克隆到puc19载体上，在大肠杆菌dh5中实现目的基因的分子克隆，经蓝白斑筛选、限制性内切酶分析、 pcr鉴定，筛选出重组阳性质粒，并对各个目的基因片段进行序列测定，从而获得ibv主要结构基因全序列。 - The results shows that the vitro expressed protein of n gene by recombinant plasmid vector in the e . coli maintains anigenicity of tgev the recombinant protein was purified acconiing to the vector self characteristic ( hisk a polyhishdine tag introduced at the amino - acid terminus of the nucleoprotein allowed for the purification of protein by nickel - chelate dsity chromataography we explored all possibilities of pedcation and gained the modified purification method . several conditions , which include diffend ph buffer and concelltheion of imidazole , were selected to purify recombinan nucleorotein
根据载体pproexhtb含有( his ) 6特点，将融合蛋白进行纯化，在纯化过程中经各项条件的探索，确定为在裂解液中含有1mmpmsf的条件下，分别经过2倍体积的buffera和bufferb洗脱后，再收集ph5 . 9 ，含有80mmol / l咪唑的1倍体积bufferc洗脱液，可得到纯化的融合蛋白。